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> Life technologies > EVOS fl auto

   
Fluorescence, brightfield and phase contrast
Patented LED light cube technology
Supports up to 4 fluorescence channels simultaneously
supports up to color and monochrome cameras
ease-of-use with X/Y scanning stage, autofocus and
  flat-focus Z-stack
Advanced imaging software (high-resolution mosaic tiling,
  multi-position well scanning, object counting, and time-lapse)
22" touch-screen LCD display, networking capability,
  and DVI output

Optics

  • Infinity-corrected
  • RMS-threaded objectives with 45 mm parfocal distance

Objective Selection

  • 2X, 0.06 NA, 6.0 mm WD
  • 4X Ph, 0.13 NA, 17.2 mm WD
  • 4X Fl, 0.13 NA, LWD
  • 10X Fl, 0.30 NA, 7.1 mm WD
  • 20X Fl, 0.45 NA, 5.9 mm WD
  • 40X Fl, 0.65 NA, 1.6 mm WD
  • 60X Fl, 0.75 NA, 1.0 mm WD
  • 4X Ph/Fl, 0.13 NA, LWD
  • 10X Ph/Fl, 0.25 NA, LWD
  • 20X Ph/Fl, 0.40 NA, LWD
  • 40X Ph/Fl, 0.65 NA, LWD
  • 60X APO Oil, 1.42 NA
  • 50x Achro objective
  • 100x Achro objective
  • 100X Fl Oil, 1.28 NA (w/ Cleaning Kit)
  • 20X Fl coverslip-corrected, 0.50 NA
  • 40X Fl coverslip-corrected, 0.75 NA

LED Light Cube Selection

  • DAPI (357 nm excitation, 447 nm emission)
  • CFP (442 nm excitation,510 nm emission)
  • GFP (470 nm excitation, 525 nm emission)
  • YFP (500 nm excitation, 542 nm emission)
  • RFP (531 nm excitation, 593 nm emission)
  • Texas Red (585 nm excitation, 624 nm emission)
  • CY5 (628 nm excitation, 692 nm emission)
  • CY7 (731 nm excitation, 825 nm emission)
  • QDots (all available wavelengths)

Illumination

  • Patented LED Light Cubes (50,000+ hour life)
  • Adjustable intensity
  • Instant ON/OFF
  • Easy install, no maintenance

Contrast Methods

  • Fluorescence and transmitted light (brightfield or phase contrast)

Dimensions

  • Height: 32.2 cm (12.7 in)
  • Width: 34.3 cm (13.5 in)
  • Depth: 47.2 cm (18.6 in)

Weight

  • 20.0 kg (44.1 lbs)

Condenser

  • 4-position turret for brightfield and phase contrast
  • Slider with diffuser block and meniscus filters

Condenser Working Distance

  • 60 mm

Stage

  • automated X/Y scanning stage
  • ravel range: 115mm x 78mm with sub-micron resolution
  • Interchangeable vessel holders for most common shapes and sizes
  • vessel holders lock down mechanism to fix sample in place during long scans

Focus mechanism

  • automated focus mechanism with sub-micron resolution

LCD Display

  • 22" high-resolution touch screen color monitor (also fully controllable via mouse), 1920 x 1080 pixel resolution

Cameras Available

  • dual monochrome and color cameras
  • monochrome camera : Sony® ICX445 monochrome CCD, 1/3" 1280 x 960 pixels, 1.3 megapixels
  • Color camera: CMOS digital camera 1360 x 1024 pixels, 3.1 megapixels

Computer

  • external PC
  • 16 GB RAM running Windows® 7 Pro
  • touch-screen monitor

Software

  • Auto exposure : adjustment for optimal exposure times
  • Autofocus : can be set up in several different modes to optimize speed and accuracy
  • Automated optical system : automated switching of parfocal objectives, LED light cubes, and monochrome/color cameras
  • Counting function : automated cell counting with watershed algorithm for easy GFP expression monitoring, live/dead assessment, and total cell count using NucBlue® Live
  • Custom pseudocolor : choose display color for each cube (monochrome camera only)
  • Freehand annotation tool : highlight areas/cells of interest using the touch-screen controls or mouse to draw freehand shapes
  • Image editing : Counting, measurements, annotation tools, and image review; automatic overlay, select/deselect channels, brightness/contrast adjustments, and saving to USB or network
  • Image stitching : capture multiple images with overlapping fields and use mosaic tiling to stitch a high-resolution image of a large area
  • Image Review : bring in images for editing or object counting
  • Manual exploration : define parameters optimal for your experiments
  • Measure and annotate : add annotations and scale bar
  • Quick Image Wizard : step-by-step guide to capture publication-quality multicolor images
  • Reduced photobleaching : variable illumination control of digital LED light source and camera signal amplification helps dramatically reduce photobleaching
  • ROI definition : define regions of interest (ROI) for scan routines
  • Routines : create, save, and run repeat routines and experiments
  • Scalebar : a scalebar can be added to a captured image via the System Basics tab
  • Scan review : zoom in/out and pan, export the entire scan or only regions of interest
  • Time lapse : use up to 8 beacons to seamlessly capture time-lapse movies on slides or microplates
  • Z-Stack flat focus : collect a series of images; extract the most "in focus" pixels from each to create a single, focused image even from thick samples

Captured Images

  • 16-bit monochrome TIFF or PNG (12-bit dynamic range)
  • 24-bit color TIFF, PNG, JPG or BMP
  • time-lapse AVI

Output Ports

  • multiple USB Ports, 1 display output with DVI adaptor (supports direct output to USB and networked storage)